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diffquik staining kit  (Sysmex Corporation)

 
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    Sysmex Corporation diffquik staining kit
    Diffquik Staining Kit, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/diffquik+staining+kit/diffquik+kit/pmc06506732-46-9-12
    Average 90 stars, based on 1 article reviews
    diffquik staining kit - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Liquid nitrogen vapor is comparable to liquid nitrogen for storage of cryopreserved human sperm: evidence from the characteristics of post-thaw human sperm.
    Article Snippet: 74 75 76 77 78 79 80 Jingmei Hu, M.D., Shidou Zhao, Ph.D., Chengyan Xu, Ph.D., Lin Zhang, M.D., Shaoming Lu, Ph.D., Linlin Cui, Ph.D., Jinlong Ma, Ph.D., and Zi-Jiang Chen, Ph.D. a Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University; b National Research Center for Assisted Reproductive Technology and Reproductive Genetics; c The Key Laboratory for Reproductive Endocrinology of Ministry of Education; d Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan; and e Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Objective: To compare the differences in the characteristics of post-thaw human sperm after storage in either liquid nitrogen (LN2; 196 C) or LN2 vapor ( 167 C).. Design: Experimental study.. Setting: University hospital.

    Article Title: β-Catenin expression is associated with cell invasiveness in pancreatic cancer
    Article Snippet: .. After incubation at 37°C, invaded cells were stained using DiffQuik staining kit (Sysmex, Kobe, Japan). ..

    Microscopy:

    Article Title: Liquid nitrogen vapor is comparable to liquid nitrogen for storage of cryopreserved human sperm: evidence from the characteristics of post-thaw human sperm.
    Article Snippet: 74 75 76 77 78 79 80 Jingmei Hu, M.D., Shidou Zhao, Ph.D., Chengyan Xu, Ph.D., Lin Zhang, M.D., Shaoming Lu, Ph.D., Linlin Cui, Ph.D., Jinlong Ma, Ph.D., and Zi-Jiang Chen, Ph.D. a Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University; b National Research Center for Assisted Reproductive Technology and Reproductive Genetics; c The Key Laboratory for Reproductive Endocrinology of Ministry of Education; d Shandong Provincial Key Laboratory of Reproductive Medicine, Jinan; and e Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 Objective: To compare the differences in the characteristics of post-thaw human sperm after storage in either liquid nitrogen (LN2; 196 C) or LN2 vapor ( 167 C).. Design: Experimental study.. Setting: University hospital.

    Incubation:

    Article Title: β-Catenin expression is associated with cell invasiveness in pancreatic cancer
    Article Snippet: .. After incubation at 37°C, invaded cells were stained using DiffQuik staining kit (Sysmex, Kobe, Japan). ..



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    Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with <t>DiffQuik</t> ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
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    diffquik staining kit  (Sysmex Corporation)
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    Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with <t>DiffQuik</t> ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
    Diffquik Staining Kit, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

    Journal: Cancers

    Article Title: ALDH1A2 Is a Candidate Tumor Suppressor Gene in Ovarian Cancer

    doi: 10.3390/cancers11101553

    Figure Lengend Snippet: Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

    Article Snippet: After 24 h, the membranes were fixed with 100% methanol and stained with a DiffQuik stain kit (Triangle Biomedical Sciences, Inc., Durham, NC, USA).

    Techniques: Expressing, Invasion Assay, Transfection, Plasmid Preparation, Staining, Microscopy, Incubation, Fluorescence